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PCR Protocols (Methods in Molecular Biology) (Methods in Molecular Biology) (Methods in...
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PCR Protocols (Methods in Molecular Biology) (Methods in Molecular Biology) (Methods in...
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by David Stirling
Sales Rank : 820997
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Hardcover: 545 pages
Publisher: Humana Press; 2 edition September 1, 2003
Language: English
ISBN-10: 0896036421
ISBN-13: 978-0896036420
Product Dimensions:
10.1 x 7.1 x 1.2 inches
Shipping Weight: 2.9 pounds
Product Review
Reviews of the First Edition:
"succeeds in covering a wider range of more general applicable methods thanits predecessors provid[es] an extensive range of versatile, expedient, and readily applicable PCR protocols."-Trends in Cell Biology
From reviews of the first edition
"useful in virtually all disciplines of the life sciencesa pleasure [to] read and use"
-American Journal of Physiology: Lung Cellular and Molecular Physiology
"an excellent resourcehas potential value for anyone looking for new ways to use PCR or new tricks to make their PCR work better."
-Biopharm
"provides an extensive range of versatile, expedient, and readily applicable PCR protocols."
-Trends in Cell Biology
"It is what all molecular biology laboratories should have and contains information that is relevant to all levels of researchers. Relevant and timely diagrams compliment the text; there are also heaps of references included at the end of each section." - Microbiologist
Product Description
In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
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